C肽对糖尿病大鼠肾脏的影响

比较C肽和胰岛素对大鼠糖尿病肾病的治疗作用。方法:选取Wistar大鼠40只,分为正常对照组(NG组)和糖尿病组(DM组),糖尿病组链脲佐菌素诱发大鼠成模后,随机分为三组:糖尿病组(DM组)、胰岛素组(IG组)和C肽组(ICG组)。治疗8周后测定各组大鼠24小时尿白蛋白排泄率(UAER)、肾重/体重,并观察糖尿病大鼠肾脏超微结构变化。结果:24小时尿白蛋白排泄率:糖尿病组明显增加,C肽组明显低于糖尿病组和胰岛素组,差异具有显著性。大鼠肾脏超微结构变化:各组大鼠肾小球截面积、肾小球平均体积(MGV)、细胞外基质/肾小球截面积比值、细胞外基质截面积、肾小球基底膜厚度相比,糖尿病组明显升高,C肽组较胰岛素组和糖尿病组明显下降,差异具有显著性。结论:C肽治疗可以降低24小时尿白蛋白排泄率,改善糖尿病大鼠肾脏超微结构病变。     

OPGmRNA在牙齿萌出过程中牙台方组织内的表达

目的：探讨骨保护素（osteoprotegerin OPG）mRNA在牙齿萌出过程中胎方组织内的表达。方法：运用原位杂交方法检测大鼠下颌第一磨牙胎方组织内OPGmRNA的表达。结果：大鼠下颌第一磨牙牙台方组织内牙囊成纤维细胞中OPGmRNA在牙齿骨内萌出阶段中期阳性表达,与萌出早、晚期比较差异有统计学意义（P〈0．01）,其中早期比晚期差异显著（P〈0．01）;成釉细胞中OPGmRNA在牙齿骨内萌出阶段中期阳性表达,与萌出早、晚期比较差异亦有统计学意义（P〈0.01）,早期与晚期没有显著性差异（P〉0．05）。结论：OPGmRNA在大鼠出生后下颌第一磨牙牙囊成纤维细胞、成釉细胞骨内萌出阶段中期表达最强,牙齿萌出过程中可能通过OPGmRNA表达量的变化来调节牙齿的萌出。     

VEGF siRNA降低人肝癌细胞株SMMC7721的致瘤性

本研究应用RNA干扰（RNAi）技术高效抑制VEGF的表达,明显降低了人肝癌细胞株SMMC7721的致瘤性。化学合成针对人VEGF基因的siRNA,体外瞬时转染人肝癌细胞株SMMC7721,RT-PCR和Elisa法检测表明VEGFsiRNA实验组与对照组相比,细胞内VEGFmRNA表达下降了76．16％,VEGF分泌蛋白表达则下降了96．28％,而MTT法结果显示VEGFsiRNA对SMMC7721细胞增殖没有明显作用。体内实验中,不同时间对荷SMMC7721细胞瘤裸小鼠进行siRNA直接瘤内注射,同时测量瘤体积,最后取瘤块进行组织切片观察并进行分子生物学分析,结果显示,与对照组相比,VEGFsiRNA实验组肿瘤体积明显缩小,瘤内出现细胞坏死,同时肿瘤组织中VEGFmRNA和蛋白表达水平均明显降低。     

腰带长体茧蜂Macrocentrus cingulum毒液和卵巢蛋白的电泳分析

寄生蜂毒液和卵巢蛋白在寄生过程中起着重要作用,蛋白成分和性质的研究日益深入.本文主要通过SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)试验,分析了腰带长体茧蜂毒液和卵巢蛋白的分子量组成.结果表明,腰带长体茧蜂毒液蛋白图谱显示约有7条蛋白带介于43～100 kDa之间,含量较高的三条带为97kDa、64kDa、45kDa;卵巢蛋白图谱显示约有12条蛋白带,位于30～200 kDa之间,含量较高的两条带为39kDa、43kDa.并对毒液和卵巢的生物学功能进行了探讨.     

Alterations of gene expression profiles induced by sulfur dioxide in rat lungs

Sulfur dioxide (SO2) is a ubiquitous air pollutant presents in low concentrations in urban air and in higher concentrations in working environment.Few data are avail-able on the effects of being exposed to this pollutant on the molecular mechanism,although some biochemical changes in lipid metabolism,intermediary metabolism and oxidative stress have been detected.The present investigation aimed at analyzing the gene expression profiles of the lungs of Wistar rats short-term (20 ppm,6 h/day,for seven days) and long.term (5 ppm,1 h/day,for 30 days) exposed to SO2 by Affymetrix GeneChip (RAE230A) analysis.It was found that 31 genes,containing 18 known genes and 13 novel genes were up-regulated,and 31 genes,containing 20 known genes and 11 novel genes,were down-regulated in rats short-term exposed to SO2 compared with control rats.While there were 176 genes,containing 82 known genes and 94 novel genes were up-regulated,and 85 genes,containing 46 known genes and 39 novel genes,were down-regulated in rats long-term exposed to SO2 compared with control rats.It is suggested that:(1) SO2 exerts its effects by different mechanisms in vivo at high-dose short-term inhalation and at low-dose long-term inhalation;(2) a notable feature of the gene expression profile was the decreased expression of genes related to oxidative phosphorylation in lungs of rats short-term exposed to SO2,which shows high-dose short-term exposed to SO2 may cause the deterioration of mitochondrial functions;(3)discriminating genes in lungs of rats long-term exposed to SO2 included those involved in fatty acid metabolism,immune,inflammatory,oxidative stress,oncogene,tumor suppresser and extracellular matrix.The mechanism of low-dose long-term exposed to SO2 is more complex.     

Characterization of novel microsatellite markers from the Yesso scallop Mizuhopecten yessoensis

Twelve polymorphic and informative microsatellite markers were isolated and characterized for the Yesso scallop, Mizuhopecten yessoensis. We characterized these loci by genotyping 48 individuals; the number of alleles ranged from two to eight, and the values of observed heterozygosity and expected heterozygosity varied from 0 to 0.8333 and from 0.2546 to 0.8231, respectively. No significant linkage disequilibrium between pairs of loci was found and three loci showed significant heterozygote deficiency from the Hardy–Weinberg equilibrium. These markers are potential for studies of the population structure, individual or hybrid identification of the species.     

用DREAM技术纠正人工合成基因中的突变

目的：介绍一种简便、有效的定点突变技术。方法：根据突变位点附近的DNA序列推导出氨基酸序列,再以此氨基酸序列进行逆翻译,这样在不改变氨基酸序列的前提下可以得到数目巨大的隐性突变体（silent mutants）,这些突变体中包含大量的限制性内切酶位点,选择合适的酶切位点设计引物用PCR技术扩增两侧DNA片段,然后以相应酶切融合这两个片段即可完成定点突变。结果：用该方法成功地在人工合成的含有缺失的可溶性组织因子基因的472位插入C,T两个碱基,校正了阅读框架,获得了预期的目的基因。结论：该方法简便、有效, 避免了多轮PCR和合成长引物导致突变的可能性,这种改进的PCR 定点诱变技术我们称之为“设计限制酶辅助突变”（Designed Restriction Enzyme Assisted Mutagenesis, DREAM）。此技术简单方便, 诱变的成功率高, 适于实验室常规应用。     

苹果属皱叶矮生型株系及其亲本和后代实生苗的过氧化物酶同工酶特性研究

以母本平邑甜茶1株、父本扎矮山定子1株、皱叶矮生型株系(F1)6株及其自然授粉后代实生苗(F2)15株为试材,采用聚丙烯酰胺凝胶电泳法研究叶片过氧化物酶同工酶谱的异同。结果表明:皱叶矮生型株系叶片的过氧化物酶同工酶酶谱共有10条酶带,R f值范围0.18~0.90,各植株间的酶带数量基本一致,只有强弱不同,与平邑甜茶酶谱的差异也很小。在皱叶矮生型株系的后代实生苗叶片中,除15 a单株有16条酶带外,其他实生苗的过氧化物酶同工酶酶谱约有10条酶带,但株间差异明显,R f值范围0.18~0.84。不同树龄平邑甜茶叶片的酶谱一致性较好。     

凤尾蕨内生真菌的研究Ⅱ——1株产芦丁内生真菌初步研究

报道了从凤尾蕨属井栏边草(Pteris multifida)根状茎中分离出一内生真菌——菌株JJF006,对其形态特征进行了初步研究,并用TLC对该菌株培养物进行了分析。初步结果表明:该真菌液体培养基中含有芦丁成分。     

红籽瓜数量性状分析软件的设计与实现

主要介绍了如何使用面向对象技术对红籽瓜数量性状分析软件的设计,阐述了软件的设计过程,代码的编写。实现了对红籽瓜数量性状的分析。